Nanomolecular Target Discovery Laboratory (TDI) focuses on the design and development of molecules for targeting purposes with main applications in infectious diseases, diabetes, and cancers. Our core strengths include (1) identification of molecular biomarkers for cancer diagnosis; (2) design and synthesis of targeting molecules e.g., antibody and its fragments, aptamers, and peptides; (3) synthesis and functionalization of nanoparticles with targeting molecules for diagnostic and therapeutic applications; and (4) development of nanosensor for medical diagnostics.
Researcher(s):
Prof. Tararaj Dharakul (Team Leader) tararaj@nanotec.or.th
Dr. Weerakanya Maneeprakorn weerakanya@nanotec.or.th
Dr. Natpapas Wiriyachaiporn natpapas@nanotec.or.th
Dr. Satita Tapaneeyakorn satita@nanotec.or.th
Dr. Suwussa Bamrungsap suwussa@nanotec.or.th
Dr. Deanpen Japrung deanpen@nanotec.or.th
Dr. Sasinee Bunyarataphan sasinee.bunyarataphan@nanotec.or.th
Dr. Tawin Iempridee tawin@nanotec.or.th
Dr. Jeerapond Leelawattanachai jeerapond@nanotec.or.th
Research Interests:
1. Dr. Weerakanya Maneeprakorn
Research interests:
(1)Inorganic nanoparticle synthesis and surface functionalization
(2) Biosensing platform using multi-modality nanoparticles for sensitive target detection
(3) MRI contrast agent based on superparamagnetic iron oxide nanoparticle for cancer diagnosis
2. Dr. Satita Tapaneeyakorn
Research interests:
(1) Protein engineering and peptide design
(2) Structural studies of protein-ligand complexes by solution- and solid-state NMRspectroscopy
(3) Screening and selection of cell-surface binding peptides for medical applications
3. Dr. Natpapas Wiriyachaiporn
Research interests:
(1) Development of nano-molecular diagnostic platforms for biosensing applications
(2)Development of enhanced sensitive and multiplex detection using lateral flowimmunochromatographic assay for rapid target detection
4. Dr. Suwussa Bamrungsap
Research interest:
(1) Fluorescence dye doped silica nanoparticle for biosensors and targeted imaging
(2) Surface enhanced Raman scattering (SERS) based nanosensors for medical diagnostic
(3) Magnetic relaxation switches (MRSw) biosensors
5. Dr. Deanpen Japrung
Research interests:
(1) Development of albumin glycation aptasensor for diabetes mellitus monitoring
(2) Identification of miRNAs as novel diagnostic biomarkers in cervical cancer and development of miRNA detection in human secretions.
(3) Nanopore technology
6. Dr. Sasinee Bunyarataphan
Research interests:
(1) Development of electrochemical aptasensor for diabetes mellitus screening
(2) Development of electrochemical nucleic acid lateral flow immunosensor for Mycobacterium tuberculosis
7. Dr. Tawin Iempridee
Research interests:
(1) Identification and characterization of circulating cancer biomarkers
(2) Molecular mechanisms of noncoding RNAs in carcinogenesis and metastasis.
(3) Development of diagnostic and therapeutic platforms for infectious diseases and cancers.
8. Dr. Jeerapond Leelawattanachai
Research interest:
(1) Self-assembled biologic nanoparticels for targeted delivery of drugs and small RNAs to tumor cells in vivo
Selected Publications:
Wiriyachaiporn, N., Maneeprakorn, W., Apiwat, C., and Dharakul, T. (2015) Dual-layered and double-targeted nanogold based immunosensor for highly sensitive detection of influenza. Microchim Acta 182, 85-93.
Apiwat, C., Wiriyachaiporn, N., Maneeprakorn, W., Dharakul, T., Thepthai, C., Puthavathana, P., Siritantikorn, S., and Horthongkham, N. (2014) Simultaneous discrimination and detection of influenza A(H1N1)pdm09 and seasonal influenza A viruses using a rapid immunogold biosensor. Archives of Virology 159(7), 1603-1611.
Bamrungsap, S., Apiwat, C., Chantima, W., Dharakul, T., and Wiriyachaiporn, N. (2014) Rapid and sensitive lateral flow immunoassay for influenza antigen using fluorescently-doped silica nanoparticles. Microchim Acta 181, 223-230.
Iempridee, T., Reusch, J. A., Riching, A., Johannsen, E. C., Dovat, S., Kenney, S.C., Mertz, J.E. (2014) Epstein-Barr virus utilizes Ikaros in regulating its latent-lytic switch in B cells. J Virol. 88(9), 4811-27.
Japrung, D., Bahrami, A., Nadzeyka, A., Peto, L., Bauerdick, S., Edel, J. B., and Albrecht, T. (2014) SSB binding to single-stranded DNA probed using solid-state nanopore sensors, The journal of physical chemistry. B 118, 11605-11612.
Japrung, D., Dogan, J., Freedman, K. J., Nadzeyka, A., Bauerdick, S., Albrecht, T., Kim, M. J., Jemth, P., and Edel, J. B. (2013) Single-molecule studies of intrinsically disordered proteins using solid-state nanopores, Analytical chemistry 85, 2449-2456.
Miles, B. N., Ivanov, A. P., Wilson, K. A., Dogan, F., Japrung, D., and Edel, J. B. (2013) Single molecule sensing with solid-state nanopores: novel materials, methods, and applications, Chemical Society reviews 42, 15-28.(Review)
Bamrungsap, S., Zhao, Z., Chen, T., Wang, L., Li, C., Fu, T., and Tan, W. (2012). Nanotechnology in therapeutics: a focus on nanoparticles as a drug delivery system. Nanomedicine 7, 1253-1271.(Review)
Japrung, D., Bahrami, A., Dogan, F., and Albrecht, T. (2012) Solid-state nanopores for biosensing with submolecular resolution, Biochemical Society transactions 40, 624-628. (Review)