Nanodiagnostics Device Research Team

Nanodiagnostics Device Research Team focuses on designing and developing screening technology systems for biomarker identification and detection at nanomolecular level. We aim to design and integrate targeting molecules with nanomaterials to develop simple, sensitive, and efficient biosensors / diagnostic / screening platform / devices in different fields of application. These applications include health & biomedical, veterinary, and drug & chemical contaminations in food & drink applications.

• Research Scope

  1. Biomarkers identification and analysis
  • Target molecule selection
  • Nanopore technology, Surface Plasmon Resonance (SPR) technology, other related techniques for target biomolecule analysis
  2. Design & fabrication of nanomaterials and biomolecules for further use in detection & screening.
  • Nanomaterials synthesis – Gold nanoparticle, Graphene oxide nanoparticle, Hybrid nanoparticle
  • Biomolecule design and fabrication – aptamers, specific peptide sequences
  3. Integration of nanomaterial and biomolecules for designing and developing screening ang detection platform technology 
  • Design and development of simple & rapid biosensors using qualitative detection platform technology – Lateral Flow Immunochromatographic Assay, LFIA (sandwich and competitive technique), Colorimetric & Fluorescence-based biosensors.
  • Design and development of sensitive and efficient biosensors using semi-quantitative detection platform technology – Electrochemical sensor, Surface Enhances Raman Scattering, SERS-based sensor, Dual E-chem & Fluorescence-based sensor

   

  Key Achievements

  1. Biomarkers identification for target molecule selection and designation
  • Sudsat, P.; Srisala, J.; Pakotiprapha, D.; Tapaneeyakorn, S.; Sritunyalucksana, K.; Thitamadee, S.; Charoensutthivarakul, S.; Itsathitphaisarn, O. VP28 Interacts with PmRab7 Irrespective of Its Nucleotide State. Rep. 2024, Nov 13,14(1):27803. https://doi.org/10.1038/s41598-024-79310-5
  • Archapraditkul, C.; Janon, K.; Japrung, D.; Pongprayoon, P. Structural and dynamic properties of urinary human serum albumin fragments: a molecular dynamics study. Biomol. Struct. Dyn. 2024, Sep;42(14), 7532-7540. https://doi.org/10.1080/07391102.2023.2240426
  • Alam, I.; Boonkoom, T.; Pitakjakpipop, H.; Boonbanjong, P.; Loha, K.; Saeyang, T.; Vanichtanankul, J.; Japrung, D. Single-Molecule Analysis of SARS-CoV-2 Double-Stranded Polynucleotides Using Solid-State Nanopore with AI-Assisted Detection and Classification: Implications for Understanding Disease Severity. ACS Appl. Bio. Mater. 2024 Feb 19, 7(2):1017-1027. https://doi.org/10.1021/acsabm.3c00998
  2. Design & fabrication of nanomaterials and biomolecules for further use in detection & screening
  • Ngamaroonchote, A.; Karn-Orachai, K. Bimetallic Au-Ag on a Patterned Substrate Derived from Discarded Blu-ray Discs: Simple, Inexpensive, Stable, and Reproducible Surface-Enhanced Raman Scattering Substrates. 2021 Jun 22, 37(24):7392-7404. https://doi.org/10.1021/acs.langmuir.1c00772
  • Apiwat, C.; Luksirikul, P.; Kankla, P.; Pongprayoon, P.; Treerattrakoon, K.; Paiboonsukwong, K.; Fucharoen, S.; Dharakul, T., Japrung, D. Graphene based aptasensor for glycated albumin in diabetes mellitus diagnosis and monitoring. Bioelectron. 2016 Aug 15, 82:140-5. https://doi.org/10.1016/j.bios.2016.04.015
  • Tapaneeyakorn, S.; Chantima, W.; Thepthai, C.; Dharakul, T. Production, characterization, and in vitro effects of a novel monoclonal antibody against Mig-7. Biophys. Res. Commun. 2016 Jun 24, 475(2):149-53. https://doi.org/10.1016/j.bbrc.2016.05.062
  3. Integration of nanomaterial and biomolecules for designing and developing screening and detection platform technology
  • Apiwat, C.; Houghton, J.W.; Ren, R.; Tate, E.; Edel, J.B.; Chanlek, N.; Luksirikul, P.; Japrung, D. Advancing Albumin Isolation from Human Serum with Graphene Oxide and Derivatives: A Novel Approach for Clinical Applications. ACS Omega. 2024 Sep 20, 9(39):40592-40607. https://doi.org/10.1021/acsomega.4c04276
  • Wiriyachaiporn, N.; Kongrueng, J.; Sukkuea, K.; Tanrattanawong, R.; Vanichtanankul, J.; Saeyang, T.; Jantra, T.; Japrung, D.; Maneeprakorn, W.; Bamrungsap, S.; Janchompoo, P.; Pasomsub, E. Characterizing a visual lateral flow device for rapid SARS-CoV-2 virus protein detection: pre-clinical and system assessment. Methods. 2024, May 3, 16(17):2740-2750. https://doi.org/10.1039/d3ay02075d
  • Pitikultham, P.; Putnin, T.; Pimalai, D.; Sathirapongsasuti, N.; Kitiyakara, C.; Jiang, Q.; Ding, B.; Japrung, D. Ultrasensitive Detection of MicroRNA in Human Saliva via Rolling Circle Amplification Using a DNA-Decorated Graphene Oxide Sensor. ACS Omega. 2023 Apr 17, 8(17):15266-15275. https://doi.org/10.1021/acsomega.3c00411
  • Karn-Orachai, K..; Ngamaroonchote, A. A label-free and selective SERS-based sensor for determination of ampicillin contamination in water using a fabric gold–silver alloy substrate with a handheld Raman spectrometer New J. Chem. 2023 47, 2758-2770. https://doi.org/10.1039/D2NJ05346B
  • Sangkaew, P.; Ngamaroonchote, A.; Sanguansap, Y.; Karn-orachai, K. Emerging Electrochemical Sensor Based on Bimetallic AuPt NPs for On-Site Detection of Hydrogen Peroxide Adulteration in Raw Cow Milk. Electrocatalysis 2022 13, 794–806. https://doi.org/10.1007/s12678-022-00763-1
  • Putnin, T.; Waiwinya, W.; Pimalai, D.; Chawjiraphan, W.; Sathirapongsasuti, N.; Japrung, D. Dual sensitive and rapid detection of glycated human serum albumin using a versatile lead/graphene nanocomposite probe as a fluorescence-electrochemical aptasensor. 2021 Jul 7, 146(13):4357-4364. https://doi.org/10.1039/d1an00556a
  • Wiriyachaiporn, N.; Sirikaew, S.; Bamrungsap, S.; Limcharoen, T.; Polkankosit, P.; Roeksrungruang, P.; Ponlamuangdee, K. A simple fluorescence-based lateral flow test platform for rapid influenza B virus screening. Methods. 2021 Apr 14, 13(14):1687-1694. https://doi.org/10.1039/d0ay01988g
  • Chawjiraphan, W.; Apiwat, C.; Segkhoonthod, K.; Treerattrakoon, K.; Pinpradup, P.; Sathirapongsasuti, N.; Pongprayoon, P.; Luksirikul, P.; Isarankura-Na-Ayudhya, P.; Japrung, D. Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor. MethodsX. 2020 Oct 20, https://doi.org/10.1016/j.mex.2020.101114